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Nanoparticles are produced in large quantities for very different applications such as lacquers, dyes, cosmetics, food, magnetic storage materials, catalysts, and print materials. On the other hand, biological systems contain different nanoparticles, e.g., liposomes. An important physical quantity of all nanoparticles is their diameter or, in the case that the nanoparticles consist of different diameters, the particle size distribution.
Reliable methods for measuring the above mentioned quantities are photometric measurements and analytical ultracentrifugation with an UV optics detector. Both methods are ruled by the Mie effect, that is scattering and absorption of the particles as function of the diameter, the wavelength, and the shape of the particles.