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Study of Interaction of Adenine with Palladium Chloride, Determination of Adenine Via Resonance Rayleigh Scattering Method (CAT#: STEM-ST-0045-YJL)

Introduction

Adenine (abbr. A), an integral base of deoxyribonucleic acid and ribonucleic acid, plays a key role in life processes of human body. It participates in synthesis of DNA and RNA in vivo, modulates activity of adenylate cyclase, controls blood flow, prevents cardiac arrhythmias, inhibits a neurotransmitter release and promotes the growth of white blood cells when they are deficiency. Adenine also participates in the intracellular energy transfer in the form of adenosine triphosphate (ATP). Therefore, adenine is not only an important vitamin of the human body, but also a clinical drug, commonly used in the prevention and treatment of arrhythmia, leucopenia and neutropenia.
The interaction of adenine with metal ions contributes to the understanding of the reaction between metal ions and nucleotide or nucleoside. Palladium chloride is used to prepare special catalysts and molecular sieves, and it can be sublimated and decomposed at 600°C. Its dihydrate is dark red hygroscopic crystal. It can be used to prepare coatings of non-conductor materials; to make gas-sensitive components, analytical reagents, etc.




Principle

Resonance Rayleigh scattering (RRS) is similar to Rayleigh scattering in nature. Resonance Rayleigh scattering is a special elastic scattering produced when the wavelength of Rayleigh scattering (RS) is located at or close to its molecular absorption band. The key to generating RRS is: when the scattering is at or close to the absorption band of the scattering molecule, since the electron absorbs the electromagnetic wave at the same frequency as the scattering frequency, the electron strongly absorbs the photon energy due to resonance and re-scatters. Its scattering intensity is several orders of magnitude higher than that of pure Rayleigh scattering, and it no longer obeys the Rayleigh law of I∝λ-4. This absorption-rescattering process is called resonance Rayleigh scattering (RRS).

Applications

Resonance Rayleigh scattering is used to the study of aggregation of chromophores on biological macromolecules and the determination of biological macromolecules such as nucleic acid, proteins and heparin, further, it has been used in the determination of trace amounts of inorganic ions and the cationic surfactant by means of ion association reactions with some dyes. In addition, it has been applied to the study of nanoparticles in liquid and the determination of β-cyclodextrin inclusion constant and the critical micelle concentration of surfactant.

Procedure

1. Sample preparation
2. Measurement by scattering detection instrument
3. Data analysis

Materials

Rayleigh scattering measurement system
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