Study of interactions between drugs and cells by Raman Spectroscopy (CAT#: STEM-ST-0004-WXH)

Introduction

The most common method for drugs to cross the cell membrane is by Passive Diffusion. Drug molecules will diffuse down its concentration gradient without expenditure of energy by the cell. However, the membranes are selectively permeable, so it has different effects on the rate of diffusion on different drug molecules.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Raman Spectroscopy is a non-destructive chemical analysis technique which provides detailed information about chemical structure, phase and polymorphy, crystallinity and molecular interactions.
The principle behind Raman spectroscopy is that the monochromatic radiation is passed through the sample such that the radiation may get reflected, absorbed, or scattered. The scattered photons have a different frequency from the incident photon as the vibration and rotational property vary.

Applications

• Analysis of biocompatibility of a material.
• Analysis of nucleic acids.
• Study of interactions between drugs and cells.
• Photodynamic therapy (PDT).
• Analyzing metabolic accumulations of a substance or compounds.
• Diagnosis of disease.
• Analysis of individual cells.
• Cell sorting applications.
• Analyzing the features of biomolecules.
• Study of bone structure.

Procedure

1. Preparation of samples
2. Determine instrument parameters
3. Perform background scan
4. Test the sample
5. Data analysis

Materials

• Raman Spectrometer
• Raman Imaging Microscope

Other recommended products

Study of saliva by Raman Spectroscopy (CAT#: STEM-ST-0070-WXH)
Study of biomembrane structure by Raman Spectroscopy (CAT#: STEM-ST-0071-WXH)
Study of atherosclerotic plaque by Raman Spectroscopy (CAT#: STEM-ST-0066-WXH)
Study of drug penetration by Raman Spectroscopy (CAT#: STEM-ST-0060-WXH)
Analysis of Triosephosphate isomerase (TPI) by Raman Spectroscopy (CAT#: STEM-ST-0018-WXH)
Non-destructive analysis of embryonic stem cells within a growing culture by Coherent Anti-Stokes Raman Spectroscopy (CARS) (CAT#: STEM-ST-0038-WXH)
Characterisation of stratum corneum by Raman spectroscopy (CAT#: STEM-ST-0040-WXH)
Characterization of DNA structures by Raman Spectroscopy (CAT#: STEM-ST-0002-WXH)