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Biofilms are developed when extracellular polymeric substances (EPS) are secreted from adherent bacterial cells to form a matrix that encloses bacterial cells. They readily adhere to biological or non-biological surfaces, are highly dynamic and heterogeneous, yet have a distinct lifecycle. The EPS accounts for the largest part of the dry mass of biofilms (up to 90%)10 and is responsible for the formation and maintenance of biofilms and their three-dimensional architecture. The matrix is a cross-linked network of polymers and has presumably multiple roles in the life of biofilms. The EPS accounts for the largest part of the dry mass of biofilms (up to 90%)10 and is responsible for the formation and maintenance of biofilms and their three-dimensional architecture. The matrix is a cross-linked network of polymers and has presumably multiple roles in the life of biofilms. P. aeruginosa is an opportunistic bacterial pathogen commonly used as a model organism for biofilm formation.