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Study of Plant Cell Wall Mechanics by Brillouin Scattering (CAT#: STEM-ST-0145-YJL)

Introduction

The plant cell wall is a natural nanoscale network structure that primarily consists of polysaccharide polymers such as cellulose, hemicellulose, and pectin, but often includes glycoproteins and lignin as well. This intricate structure encases plant cells and provides them with diverse shapes, sizes, and distinct physicochemical properties that allow them to fulfill their roles in different organs and at various developmental stages. The plant cell wall plays many fundamental roles, including determining plant morphogenesis and architecture, providing mechanical support for the plant body, conducting water and nutrients, defending against biotic and abiotic stresses, and so on. Plant cell walls are highly complex structures, which are adaptable to various environmental stresses. While many biochemical pathways have been discovered, cellular stress indicators are widely unknown.




Principle

From a quantum point of view, Brillouin scattering is an interaction of light photons with acoustic or vibrational quanta (phonons), with magnetic spin waves (magnons), or with other low frequency quasiparticles interacting with light. The interaction consists of an inelastic scattering process in which a phonon or magnon is either created (Stokes process) or annihilated (anti-Stokes process). The energy of the scattered light is slightly changed, that is decreased for a Stokes process and increased for an anti-Stokes process. This shift, known as the Brillouin shift, is equal to the energy of the interacting phonon and magnon and thus Brillouin scattering can be used to measure phonon and magnon energies.

Applications

Brillouin scattering is used to determine acoustic velocities and elastic properties of a number of crystalline solids, glasses, and liquids.

Procedure

1. Sample preparation
2. Measurement by scattering detection instrument
3. Data analysis

Materials

Brillouin scattering measurement system (Brillouin spectrometer)