Study of metal ion labeling of the conformational and charge states of lysozyme by ion mobility mass spectrometry (CAT#: STEM-ST-0132-LJX)
Introduction
Lysozyme is a protein-based defense factor, an important immune factor of animals, and a hydrolytic enzyme that acts specifically on microbial cell wall, also known as cell wall lysin.
Ion mobility spectrometry–mass spectrometry (IMS-MS) is an analytical chemistry method that separates gas phase ions based on their interaction with a collision gas and their masses. In the first step, the ions are separated according to their mobility through a buffer gas on a millisecond timescale using an ion mobility spectrometer. The separated ions are then introduced into a mass analyzer in a second step where their mass-to-charge ratios can be determined on a microsecond timescale.
Applications
For studying the gas phase ion structure For detecting the chemical warfare agents and explosives For the analysis of proteins, peptides, drug-like molecules and nano particles For monitoring isomeric reaction intermediates and probe their kinetics For proteomics and pharmaceutical analysis
Procedure
1. Add sample 2. The ions in the sample are separated in the ion mobility spectrometer 3. The separated ions are introduced into the mass analyzer for detection 4. Store the detection results
Materials
• Sample Type: Lysozyme
Notes
1. Ion mobility spectrometry is also a very fast technique, making it suitable for high-throughput applications. The entire analysis can be completed in just a few minutes. 2. The method is extremely sensitive and able to detect trace amounts of contaminants that other spectrometry methods would miss. 3. The effective separation of analytes achieved with this method makes it widely applicable in the analysis of complex samples such as in proteomics and metabolomics.