Unlock Exclusive Discounts & Flash Sales! Click Here to Join the Deals on Every Wednesday!

Study of Natural Porphyrins by Raman Spectroscopy (CAT#: STEM-ST-0076-WXH)

Introduction

Porphyrins are a class of cyclic aromatic molecules containing four pyrrole groups, which can form complexes with metal atoms and occur in many naturally occurring biological systems, often serving roles as sensors. The most notable of these is heme, an iron-conjugated porphyrin that is found in hemoglobin in blood cells, and the metalloprotein cytochrome c.




Principle

Raman Spectroscopy is a non-destructive chemical analysis technique which provides detailed information about chemical structure, phase and polymorphy, crystallinity and molecular interactions.
The principle behind Raman spectroscopy is that the monochromatic radiation is passed through the sample such that the radiation may get reflected, absorbed, or scattered. The scattered photons have a different frequency from the incident photon as the vibration and rotational property vary.

Applications

• Analysis of biocompatibility of a material.
• Analysis of nucleic acids.
• Study of interactions between drugs and cells.
• Photodynamic therapy (PDT).
• Analyzing metabolic accumulations of a substance or compounds.
• Diagnosis of disease.
• Analysis of individual cells.
• Cell sorting applications.
• Analyzing the features of biomolecules.
• Study of bone structure.

Procedure

1. Preparation of samples
2. Determine instrument parameters
3. Perform background scan
4. Test the sample
5. Data analysis

Materials

• Raman Spectrometer
• Raman Imaging Microscope
Advertisement