Study of Type IB Topoisomerases by Magnetic tweezers (MT) (CAT#: STEM-MB-1245-WXH)

Introduction

Type IB DNA topoisomerases are found in all eukarya, two families of eukaryotic viruses (poxviruses and mimivirus), and many genera of bacteria. They alter DNA topology by cleaving and resealing one strand of duplex DNA via a covalent DNA-(3-phosphotyrosyl)-enzyme intermediate.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Magnetic tweezers (MT) are scientific instruments for the manipulation and characterization of biomolecules or polymers. These apparatus exert forces and torques to individual molecules or groups of molecules. It can be used to measure the tensile strength or the force generated by molecules.

Applications

Study of mechanical properties of biological macromolecules like DNA or proteins in single-molecule experiments.
Study of the rheology of soft matter.
Study of force-regulated processes in living cells.

Procedure

1.Sample preparation
2.Force Calibration
3.Measurement
4.Analysis

Materials

Magnetic tweezers

Other recommended products

Study of DNA packing by a cationic lipid DSTAP by Magnetic tweezers (MT) (CAT#: STEM-MB-1268-WXH)
Nanomechanics of Fluorescent DNA Dyes on DNA by Magnetic tweezers (MT) (CAT#: STEM-MB-1259-WXH)
Study of DNA-Protein Interactions by Magnetic tweezers (MT) (CAT#: STEM-MB-1273-WXH)
Study of the dynamic activity of helicases by Magnetic tweezers (MT) (CAT#: STEM-MB-1234-WXH)
Analysis of Force Spectroscopy by Magnetic tweezers (MT) (CAT#: STEM-MB-1258-WXH)
Study of the elastic properties of DNA by Magnetic tweezers (MT) (CAT#: STEM-MB-1232-WXH)
Study of DNA supercoiling by Magnetic tweezers (MT) (CAT#: STEM-MB-1274-WXH)
Measurement of the Force–Velocity Relationship for Multiple Kinesin Motors by Magnetic tweezers (MT) (CAT#: STEM-MB-1262-WXH)