Study of the dynamic activity of recombinases by Magnetic tweezers (MT) (CAT#: STEM-MB-1236-WXH)

Introduction

Recombinases are enzymes that catalyse site-specific recombination events within DNA; for example, genetic recombination during meiosis in which recombination serves to generate new combinations of alleles on chromosomes.

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Principle Applications Procedure Materials Notes Related Products

Principle

Magnetic tweezers (MT) are scientific instruments for the manipulation and characterization of biomolecules or polymers. These apparatus exert forces and torques to individual molecules or groups of molecules. It can be used to measure the tensile strength or the force generated by molecules.

Applications

Study of mechanical properties of biological macromolecules like DNA or proteins in single-molecule experiments.
Study of the rheology of soft matter.
Study of force-regulated processes in living cells.

Procedure

1.Sample preparation
2.Force Calibration
3.Measurement
4.Analysis

Materials

Magnetic tweezers

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