Study of the dynamic activity of topoisomerases by Magnetic tweezers (MT) (CAT#: STEM-MB-1235-WXH)

Introduction

Topoisomerases alter DNA topology by binding to the DNA, cleaving either one or both strands of the double helix, then (for most of these enzymes) passing either the other strand of the same helix or another double strand through the break, and finally resealing the DNA backbone.

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Principle Applications Procedure Materials Notes Related Products

Principle

Magnetic tweezers (MT) are scientific instruments for the manipulation and characterization of biomolecules or polymers. These apparatus exert forces and torques to individual molecules or groups of molecules. It can be used to measure the tensile strength or the force generated by molecules.

Applications

Study of mechanical properties of biological macromolecules like DNA or proteins in single-molecule experiments.
Study of the rheology of soft matter.
Study of force-regulated processes in living cells.

Procedure

1.Sample preparation
2.Force Calibration
3.Measurement
4.Analysis

Materials

Magnetic tweezers

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