Measurement of the Stall Torque of the Flagellar Motor in Escherichia coli by Magnetic tweezers (MT) (CAT#: STEM-MB-1260-WXH)

Introduction

The flagellar motor drives the rotation of flagellar filaments, propelling the swimming of flagellated bacteria. The maximum torque the motor generates, the stall torque, is a key characteristic of the motor function.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Magnetic tweezers (MT) are scientific instruments for the manipulation and characterization of biomolecules or polymers. These apparatus exert forces and torques to individual molecules or groups of molecules. It can be used to measure the tensile strength or the force generated by molecules.

Applications

Study of mechanical properties of biological macromolecules like DNA or proteins in single-molecule experiments.
Study of the rheology of soft matter.
Study of force-regulated processes in living cells.

Procedure

1.Sample preparation
2.Force Calibration
3.Measurement
4.Analysis

Materials

Magnetic tweezers

Other recommended products

Study of Receptor-ligand interactions Using Magnetic Tweezers (CAT#: STEM-MB-1243-WXH)
Study of mechanosensing proteins by Magnetic tweezers (MT) (CAT#: STEM-MB-1244-WXH)
Study of the activities of conformational deformed enzyme by Magnetic tweezers (MT) (CAT#: STEM-MB-1257-WXH)
Study of Type IB Topoisomerases by Magnetic tweezers (MT) (CAT#: STEM-MB-1245-WXH)
Study of the Viscoelastic Properties of Fibrin Clots by Magnetic tweezers (MT) (CAT#: STEM-MB-1272-WXH)
Study the Binding of anti-cancer chemotherapeutic drug mitoxantrone to DNA by Magnetic tweezers (MT) (CAT#: STEM-MB-1256-WXH)
Study of DNA supercoiling by Magnetic tweezers (MT) (CAT#: STEM-MB-1274-WXH)
Study of the dynamic activity of polymerases by Magnetic tweezers (MT) (CAT#: STEM-MB-1233-WXH)