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Measurement of torsional stiffness in RecA-DNA filaments by Magnetic tweezers (MT) (CAT#: STEM-MB-1277-WXH)

Introduction

RecA protein, which plays an essential role in homologous recombination in Escherichia coli, polymerizes around single-stranded DNA (ssDNA) and forms a right-handed helical filamentous structure.1., 2., 3., 4. It promotes a search for homology between ssDNA and double-stranded DNA (dsDNA), and exchanges their homologous strands to form heteroduplex DNA.

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Principle Applications Procedure Materials

Principle

Magnetic tweezers (MT) are scientific instruments for the manipulation and characterization of biomolecules or polymers. These apparatus exert forces and torques to individual molecules or groups of molecules. It can be used to measure the tensile strength or the force generated by molecules.

Applications

Study of mechanical properties of biological macromolecules like DNA or proteins in single-molecule experiments.
Study of the rheology of soft matter.
Study of force-regulated processes in living cells.

Procedure

1.Sample preparation
2.Force Calibration
3.Measurement
4.Analysis

Materials

Magnetic tweezers

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