The Study of Interaction of Gold Nanoparticles with Berberine Hydrochloride by Resonance Rayleigh Scattering (CAT#: STEM-ST-0023-YJL)

Introduction

Gold nanoparticles attract great scientific and technological interest because of their novel physical and chemical properties. The chemical modified gold nanoparticle, which is used as optical probe and chemical sensor in the field of analytic chemistry has been widely studied. As a RRS probe, gold nanoparticle not only has more advantages such as dispersed, homogeneous and stable than other metal nanoparticle (Ag, Cu, etc.), but the products of gold nanoparticle binding with biomacromolecules have better analytical properties.
Berberine hydrochloride (BBH)is an isoquinoline alkaloid and exists as a quaternary ammonium cation in aqueous solution. Natural berberine is mainly contained in coptics plants and usually exists as a hydrochloride or sulfate.It is a medicine used for antibiosis and inflammation and is also a natural drug with low poison danger and low side effects.

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Principle Applications Procedure Materials Notes Related Products

Principle

Resonance Rayleigh scattering (RRS) is similar to Rayleigh scattering in nature. Resonance Rayleigh scattering is a special elastic scattering produced when the wavelength of Rayleigh scattering (RS) is located at or close to its molecular absorption band. The key to generating RRS is: when the scattering is at or close to the absorption band of the scattering molecule, since the electron absorbs the electromagnetic wave at the same frequency as the scattering frequency, the electron strongly absorbs the photon energy due to resonance and re-scatters. Its scattering intensity is several orders of magnitude higher than that of pure Rayleigh scattering, and it no longer obeys the Rayleigh law of I∝λ-4. This absorption-rescattering process is called resonance Rayleigh scattering (RRS).

Applications

Resonance Rayleigh scattering is used to the study of aggregation of chromophores on biological macromolecules and the determination of biological macromolecules such as nucleic acid, proteins and heparin, further, it has been used in the determination of trace amounts of inorganic ions and the cationic surfactant by means of ion association reactions with some dyes. In addition, it has been applied to the study of nanoparticles in liquid and the determination of β-cyclodextrin inclusion constant and the critical micelle concentration of surfactant.

Procedure

1. Sample preparation
2. Measurement by scattering detection instrument
3. Data analysis

Materials

Rayleigh scattering measurement system

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