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Thioredoxins Analysis in Plant Tissues

Thioredoxins (TRXs) belongs to a family of phylogenetically related oxidoreductases. TRXs in plant are involved in the control of plant metabolism, development, phytohormone pathways, and responses to abiotic and biotic stressors. Accumulated evidence endorses the prominent role of TRXs as a key component in regulation of plant immune signaling. TRX activity also plays a role in local and systemic immune signaling by the immune hormone SA.

Pathogen infection induces accumulation of ROS and RNS which are not only important in cell signaling, but also lead to undesired oxidative thiol modifications of antioxidant enzymes, such as monodehydroascorbate reductases (MDAR), dehydroascorbate reductases (DHAR). A growing body of evidence now suggests that TRX enzymes may be involved in protecting antioxidant enzymes from oxidative damage or inhibition. In addition, TRXs can supply reducing power to reductases, which are involved in the degradation of lipid hydroperoxides or reparation of oxidized proteins, including peroxiredoxins, glutathione peroxidases, and methionine sulfoxide reductases.

 Fig. 1 Signaling and protective functions of TRX enzymes in plant immunity Fig. 1 Signaling and protective functions of TRX enzymes in plant immunity.

STEMart provides comprehensive analysis services to identify and investigate the function of TRX in regulating plant environmental stress responses.

Test Samples

Plant tissue

Test Capabilities

  • Identification of TRX genes
  • Phylogenetic analysis of TRX genes
  • Structure analysis of TRX genes
  • Expression profile of TRX genes of plant under stress conditions
  • 3D structure and physicochemical properties of TRX genes
  • Subcellular localization of TRX enzymes and Isoenzymes analysis
  • Activity determination of TRX

Why Choose Us

  • Comprehensive test report including relevant experimental steps, relevant parameters of the experiment, original pictures, original data, and complete experiment results
  • Customized service for plant research
  • Professional instruments and equipment for reliable data and results
  • Rapid turnaround and cost-effective

For more information about our plant TRX analysis service, please contact us.

References

  1. Dos Santos C.V., Rey P. (2006). “Plant thioredoxins are key actors in the oxidative stress response”. Trends Plant Sci.11:329–334.
  2. Capilla Mata-Pérez, Steven H. Spoel. (2019). “Thioredoxin-mediated redox signalling in plant immunity”. Plant Science. Pages 27-33

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