Zinc Chelating Resin, 10ml Resin, 786-287, G-BIOSCIENCES (CAT#: STEM-C-3201-LGZ)

Immobilized metal ion affinity chromatography (IMAC) was developed by Porath and is based on the interaction of certain protein residues (histidine, cysteine and to some extent tryptophan) with transition metal cations effect. Zinc chelating resins are specifically designed for the purification of proteins associated with zinc ions, including 6-fold histidine-tagged proteins.

Immobilized metal affinity chromatography (IMAC) resin utilizes zinc (Zn2+) to purify 6-fold histidine-tagged proteins.

This resin binds six histidine residues (6x His), a common tag used for protein purification. The resin consists of iminodiacetate coupled to 6% cross-linked agarose beads. The binding capacity of iminodiacetic acid and divalent zinc ions is 20~40μmol/ml resin. The protein binding capacity is 20 mg protein per ml resin. We have demonstrated the binding of 40mg of 50kDa 6XHis-tagged protein to one milliliter of resin.

Immobilized nickel, cobalt and copper chelating resins are also available. Cobalt has the highest selectivity, followed by zinc, nickel, and copper, but has the lowest loading capacity. Copper has the highest loading capacity, followed by nickel and then zinc.

Specific binding/wash and elution buffers are available.

Size: 10ml Resin
High capacity: >20mg/ml
Ligand density: 20-40μmoles Zn2+ /ml resin
Bead Structure: 6% cross-linked agarose

His Binding/ Wash Buffer (10mM Imidazole, 300mM NaCl, 50mM Sodium Phosphate (pH8.0)), 100ml, 786-542, G-BIOSCIENCES (CAT#-STEM-C-3181-LGZ)
His Elution Buffer (250mM Imidazole, 300mM NaCl, 50mM Sodium Phosphate (pH8.0)), 100ml, 786-543, G-BIOSCIENCES (CAT#-STEM-C-3182-LGZ)
Ni-NTA Magnetic Beads, 1ml, 786-910, G-BIOSCIENCES (CAT#-STEM-C-3183-LGZ)
Ni-NTA Magnetic Beads, 5ml, 786-911, G-BIOSCIENCES (CAT#-STEM-C-3184-LGZ)
Nickel Chelating Resin, 10ml Resin, 786-281, G-BIOSCIENCES (CAT#-STEM-C-3185-LGZ)
Nickel Chelating Resin, 0.2ml Spin Column, 25 Columns, 786-392, G-BIOSCIENCES (CAT#-STEM-C-3186-LGZ)