Condition: Refurbished, Certified Pre-owned
Solvent delivery system:
Configuration :
Isocratic, PV5 ternary or quaternary, DR5 binary or ternary
Ranges
Pressure: 0-400 bar (0-5880 psi).
Flow: 0-5000 µl/min, in 1µl/minsteps
Flow Accuracy: +2% of setpoint or +1 µl/min
Whichever is greater, independent of back pressure and solvents, excluding volume contraction effects
Precision: <0.3% RSD, based on retention time
Composition
Precision PV5: +0.25% absolute, peak to peak, water/acetonitrile, 500µl/min, 2500 µl/min
Precision PV5: +0.15% absolute, peak to peak, water/acetonitrile, 50µl/min, 2500 µl/min
Delay Volume
PV5: 1750-2000 µl maximum, dependent on back pressure
DR5: 300-500 µl maximum, dependent on back pressure
Heated Compartment
Solvent and samples are preheated in low-volume (2µl) heat exchanger with optional cooling for sub-ambient operation
Settable Temperature range: 0-100°C in 0.1°C steps.
Controlled temperature range: 20 °C above ambient to 100°C
Temperature stability: +0.5 °C
Injection system
Maximum pressure: 400 bar (5880 psi)
Manual Injector
Rheodyne valves 7125 or 7413, with loops from 0.5 µl to 100 µl
Auto-injector
Injection volume: -25 µl (standard), or 0.1-250 µl (optional), in 0.1 µl steps
Precision
<1% (typically 0.6%) RSD from 2-25 µl (25 µl syringe);
<1% (typically 0.6%) RSD from 10-250 µl (250 µl syringe)
Sample vials: 0 mL standard, 300 disposable micovial, 100 µl microvial
Minimum Sample volume: 40 µl (2 mL standard vial), 20 µl (300 µl disposable microvial), 5 µl (100 µl microvial)
Auto-sampler: 10 removable magazines with 10 vials each (standard or microvials), with random access time < 10 seconds from any position.
Filter-photometric detector
Filters
Interference: 254 nm standard, 210,230,269,280,340,430 and 540 nm optional.
Bandwidth: 10 nm typically.
Signal Characteristics
Noise: 5x 10 E-5 AU
Drift: <1 x 10 E-3 AU/hour
Signal Ranges
Linear absorbance range: -0.1 to 1.5 AU
Response time: 100,400 and 800(10-90%)
Flow Cell
Stainless steel cell with 4.5 µl volume, 6 mm path length and maximum pressure of 100 bar (1470 psi).
Diode-array detector
Up to eight signals with definable sample and reference wavelength and bandwidth
Signal Characteristics
Noise: +3.5 x 10 E-5 AU
Drift: <2 x 10 E-3 AU/hour
Signal ranges
Wavelength range: 190 – 600 nm, in steps of 1 nm
Bandwidth range: 4-400 nm, in stepsof 1 nm
Linear absorbance range: -0.1 to 1.5AU
Spectral ranges
Wavelength range: 190 – 600 nm
Linear absorbance range: -0.1 to 1.5AU
Scan Time: 10 msec from 190-600 nm
Flowcells
Standard cell: 8 µl, 6 mm, max pressure 120 bar
High sensitivity cell: 13 µl, 10 mm,max pressure 120 bar
Slits: 2,4, and 8 nm selectable