Analysis Biomolecular Interactions of Protein-Nucleic Acid by BLI (CAT#: STEM-MB-0088-CJ)

Introduction

Proteins are the “workhorses” of the cell, participating in every structure and function involved in life. The human genome contains genes encoding 500,000 different types of proteins, of which 10,000 are expressed in the cell at any given time. It is estimated that almost 80% of these cellular proteins function in complexes rather than in isolation. Proteins interact with other proteins or biomolecules such as nucleic acids (DNA or RNA), metabolites, or lipids in order to serve as catalysts, defenses against foreign pathogens, mechanical supports, transporters, pumps, switches, growth factors, signal transduction receptors, and motors, among other functions. Characterization of protein-protein and protein-ligand interactions through analysis of real-time kinetic and binding affinity data can provide unique insights into specific molecular interactions that regulate cellular processes, and characterization of aberrant molecular interactions may provide insights into pathway dysregulation and disease.