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S-peptide (residues 1-20) and S-protein (residues 21-124) are the enzymatically inactive products of the limited digestion of ribonuclease A by subtilisin. S-peptide binds S-protein with high affinity to form ribonuclease S, which has full enzymatic activity. Biotinylated S-peptide A20G was synthesized on pre-loaded Fmoc-Gly-Wang resin (0.2 mmol).