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Glycolipids are a type of complex lipids comprising carbohydrates, fatty acids, sphingolipids or a glycerol group. Glycolipids are found on the outer leaflet of cellular membranes where it plays not only a structural role to maintain membrane stability but also facilitates cell-cell communication acting as receptors, anchors for proteins and regulators of signal transduction. Shiga toxin (Stx) producing E. coli (STEC) is a major cause of foodborne illnesses. Themajor virulence factor of STEC is Stx, an AB5 toxin that consists of a single A-subunit with ribosomal RNA-cleaving activity, surrounded by a receptor-binding B-penta mer. Two major isoforms, Stx1 and Stx2, and Stx2 variants (Stx2a-h) significantly differ in toxicity.STEC colonize the gastrointestinal tract and produce Stx.