Analysis of AGP (Human) by ELISA (CAT#: STEM-MB-0652-LGZ)

Introduction

α1-acidglycoprotein (α1-acidglycoprotein, AGP or AAG), called serum mucoid (ORM) in the early stage, is a non-specific acute reaction protein, It is also the glycoprotein with the most sugar content (about 45% sugar content) and the most acidic (PI 2.7 ~ 3.5) in human serum. It is mainly produced by liver macrophages and granulocytes. Later studies have found that cancer cells can also be synthesized. AGP serum concentrations can be affected by certain physiological and pathological conditions, such as pregnancy, burns, certain medications, and specific diseases, especially HIV infection. With the increase of age, AGP serum concentration also decreased.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Metabolic, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, Plasma

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