Analysis of ALK Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1129-LGZ)

Introduction

Official Full Name: ALK receptor tyrosine kinase
Also known as: ALK1; CD246; NBLST3
This gene encodes a receptor tyrosine kinase that belongs to the insulin receptor superfamily. The protein consists of an extracellular domain, a hydrophobic stretch corresponding to a single-pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and affects specific neurons in the nervous system. The gene has been found to be rearranged, mutated or amplified in a range of tumors, including anaplastic large cell lymphoma, neuroblastoma and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumorurigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosomal some 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19) , and ALK/MSN (chromosome X).

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Hereditary cancer-predisposing syndrome

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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