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Analysis of BCL Ag by Real-Time PCR Method (CAT#: STEM-MB-2931-LGZ)

Introduction

A real-time polymerase chain reaction (real-time PCR,or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time),not at its end,as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter,which permits detection only after hybridization of the probe with its complementary sequence.




Principle

Brucellosis is a zoonotic infectious disease caused by Brucella. Brucellosis often occurs in cattle, sheep and pigs. Infected livestock are the main source of human brucellosis.
In this experiment, specific primers and molecular beacon probes were designed for brucella genes. Under the condition that brucella DNA was contained in the samples to be tested, PCR reaction was carried out and fluorescence signals were released. The instrument was used to monitor and output the corresponding fluorescence signal in the process of PCR, and the qualitative analysis of the detection results was realized.

Applications

Used for qualitative screening and detection of brucella.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

• Sample Type: Venous blood/colony/moss, etc