Analysis of Bovine Rhinitis A Virus (BRAV) by Real-Time PCR Method (CAT#: STEM-MB-2964-LGZ)

Introduction

Bovine rhinitis virus (BRV) is an important pathogen causing bovine respiratory disease complex (BRDC) and it can be divided into two genotypes—bovine rhinitis A virus (BRAV) and bovine rhinitis B virus (BRBV).
A real-time polymerase chain reaction (real-time PCR,or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.
Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.