Analysis of C8G Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2011-LGZ)

Introduction

Official Full Name: complement C8 gamma chain<br />Also known as: C8C<br />The protein encoded by this gene belongs to the lipocalin family. It is one of three subunits that make up complement component 8 (C8), which consists of a disulfide-linked C8 α-γ heterodimer and a non-covalently associated C8 β chain. C8 is involved in the formation of the membrane attack complex (MAC) on the bacterial cell membrane. While subunits α and β play a role in complement-mediated bacterial killing, the γ subunit is not required for bactericidal activity.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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