Analysis of CD40L (soluble) (Human) by ELISA (CAT#: STEM-MB-0729-LGZ)

Introduction

CD40L, also known as gp39, TNF associated activation protein (TRAP), and T cell-B cell-activating molecule (T-BAM), Is a 33kD type II transmembrane glycoprotein with a total of 266aa, including 214 amino acids in the extracellular region, which has high homology with TNF at the amino acid level.<br />CD40L was mainly expressed in activated CD4+T lymphocytes, but also in partially activated CD8+T cells, basophil, mast cells and NK cells. CD40L was also expressed in thymus tumor cell line EL-4. CD3 mab can stimulate T cells to express CD40L.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or cell culture supernatant

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