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Analysis of DCN/Decorin by ELISA (CAT#: STEM-MB-1540-LGZ)

Introduction

Decorin (Core proteoglycans) is a secreted proteoglycan of the leucine-rich low molecular proteoglycan (SLRP) family. Decorin regulates the biological activity of extracellular collagen matrix as well as matrix-related growth factors FGF, GDF-8 / Myostatin, TGF-beta, and WYP-1. It also binds and activates the EGF receptor, ErbB4 and IGF-1 receptors. Decorin promotes myoblast differentiation, supports angiogenesis and inhibits tumor development. Some studies have shown that Decorin may inhibit the contracture of hypertrophic scar, indicating a certain role in preventing the formation of hypertrophic scar and promoting the maturation of scar.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples

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