Analysis of DLL1 by ELISA (CAT#: STEM-MB-1521-LGZ)

Introduction

DLL1 (Delta-like protein 1, delta-like protein 1) is a transmembrane glycoprotein of Notch ligand. It plays an important role in the morphogenesis of the central nervous system, the formation of intervertebral joints, the development and formation of hair cells in the inner ear, and the formation of arteries. DLL1 is involved in regulating homeostasis and differentiation of nerve, pancreatic and muscle progenitor cells, T lymphocytes and B lymphocytes. DLL1 is overexpressed in many cancers and is associated with tumorigenesis. The extracellular domain of DLL1 can be shed from the cell surface, and the intracellular domain can be cleaved by gamma-secretory enzymes and transferred to the nucleus to regulate gene transcription.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples

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