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Analysis of Elastase-2 (Human) by ELISA (CAT#: STEM-MB-0777-LGZ)

Introduction

Elastase 2 is a chymotrypsin family serine protease that plays an important role in pathogen clearance. It is expressed by promyelocytes and stored in intracellular azurophilic granules of polymorphonuclear leukocytes. ELA2 can degrade a variety of extracellular matrix proteins, including elastin and fibronectin. Multiple mutations in the ELA2 gene cause severe congenital and cyclic neutropenia.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

For the determination of Human Elastase-2.

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Cell culture media, Cell Lysate, Cit plasma, EDTA Plasma, Hep Plasma, Serum
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