Analysis of Extracellular Vesicles by Nanoparticle tracking analysis (NTA) (CAT#: STEM-MB-0714-WXH)

Introduction

Extracellular vesicles (EVs) are subcellular fragments that originate from the endosomal compartment or are shed from the plasma membrane of virtually all cell types of the human body under both, physiological and pathological conditions. They have been recognized as major players in intercellular communication and can exhibit variable functions, depending on their cellular origin, their membrane composition, and surface-associated proteins, as well as their cargo.

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Principle Applications Procedure Materials Notes Related Products

Principle

Nanoparticle tracking analysis (NTA) is a method for visualizing and analyzing particles in liquids that relates the rate of Brownian motion to particle size. The rate of movement is related only to the viscosity and temperature of the liquid; it is not influenced by particle density or refractive index. NTA allows the determination of a size distribution profile of small particles with a diameter of approximately 10-1000 nanometers (nm) in liquid suspension.

Applications

NTA has been used by commercial, academic, and government laboratories working with nanoparticle toxicology, drug delivery, exosomes, microvesicles, bacterial membrane vesicles, and other small biological particles, virology and vaccine production, ecotoxicology, protein aggregation, orthopedic implants, inks and pigments, and nanobubbles.

Procedure

1. Sample preparation
2. Nanoparticle Tracking Analysis
3. NTA Data Processing, Display, and Interpretation

Materials

• Nanoparticle Tracking Analysis (NTA) instrument
• NanoSight instrument
• Nanoparticle Analyzer

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