Analysis of Fc-epsilon RII (Human) by ELISA (CAT#: STEM-MB-0807-LGZ)

Introduction

Fc epsilon receptor (FcεR) is the Fc receptor that binding immunoglobulin E (IgE). There are two types of FcεRs, called Fc epsilon RI (FcεRI) and Fc epsilon receptor II (FcεRII/CD23). FcεRII/CD23 is the low-affinity receptor for immunoglobulin (Ig)E and plays important roles in the regulation of IgE responses. FcεRII/CD23 is expressed in T and B lymphocytes, polymorphonuclear leucocytes, monocytes, follicular dendritic cells, intestinal epithelial cells and bone marrow stromal cells, and its expression to subject to regulation by a number of stimuli.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Intracellular Signaling

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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