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Analysis of Vitronectin/VTN by ELISA (CAT#: STEM-MB-1578-LGZ)

Introduction

Vitronectin (VTN), a member of the chymosin family, is abundant in serum, extracellular matrix and bone. It is a secreted protein that exists as a single chain or as a sandwich, double chain formed by disulfide bonds. VTN consists of three domains: an N-terminal somatomodulin B domain, a central domain with homology to heme, and a C-terminal domain. VTN promotes cell adhesion and spreading, inhibits membrane disruption of terminal cellular complement pathways, and binds to various serine protease inhibitors.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

It is speculated that VTN is related to hemostasis, cell migration and malignancy.

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples
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