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Analysis of Osteoactivin (Human) by ELISA (CAT#: STEM-MB-1104-LGZ)

Introduction

Osteoactivin (OA), also known as transmembrane glycoprotein HGFIN, DC-HIL and NMB (GPNMB), belongs to the PMEL/NMB family. Osteoactivin contains a PKD domain. Osteoactivin is a transmembrane glycoprotein that is upregulated in several cancer cells, including glioblastoma multiforme, in many melanoma cells, and in tissue macrophages. Osteoactivin protein is a downstream mediator of BMP-2 affecting osteoblast differentiation and function. Osteoactivin is involved in bone mineralization and acts as a negative regulator of macrophage inflammation.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Kidney Injury, Toxicology

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Cell culture supernatant, Plasma, Serum

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