Analysis of FCoVs in Feline Infectious Peritonitis (FIP) by RT-qPCR (CAT#: STEM-MT-0069-LGZ)

Introduction

Feline infectious peritonitis (FIP) is a fatal disease of cats, a sequela of systemic feline coronavirus (FCoV) infection. Mutations in the viral spike (S) gene were associated with fCoV found in tissues from cats with FIP but not in feces from healthy cats, and were associated with monocyte/macrophage tropism and systemic dissemination.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Feline Infectious Peritonitis (FIP)

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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