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Analysis of gelatin/silver nanoparticle antimicrobial composite films by UV-Vis Spectroscopy (CAT#: STEM-MB-0922-WXH)

Introduction

Among the metal NPs, silver nanoparticles (AgNPs) have attracted especial attention in the food packing sector because of their remarkable and broad spectrum of antimicrobial effect against food-borne pathogens. The incorporation of AgNPs into the food packaging system could effectively inhibit the growth of pathogenic microorganisms.
Gelatin is an animal protein obtained by a controlled hydrolysis of the fibrous insoluble collagen from bones and skins of animals and fish. It is well known for its film-forming properties. In addition, it is abundantly available, with low cost, and is easily biodegradable and biocompatible. Gelatin films have excellent barrier properties against gas, oxygen and aromas at low or intermediate relative humidity. Gelatin has frequently been used as a suitable raw material to make edible or biodegradable films for effective food packaging.




Principle

UV-Vis spectroscopy is an analytical technique that measures the amount of discrete wavelengths of UV or visible light that are absorbed by or transmitted through a sample in comparison to a reference or blank sample. This property is influenced by the sample composition, potentially providing information on what is in the sample and at what concentration. The only requirement is that the sample absorb in the UV-Vis region, i.e. be a chromophore. Absorption spectroscopy is complementary to fluorescence spectroscopy. Parameters of interest, besides the wavelength of measurement, are absorbance (A) or transmittance (%T) or reflectance (%R), and its change with time.

Applications

UV/Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of diverse analytes or sample, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules. Spectroscopic analysis is commonly carried out in solutions but solids and gases may also be studied.

Procedure

1. Calibrate the Spectrometer
2. Perform an Absorbance Spectrum
3. Kinetics Experiments with UV-Vis Spectroscopy

Materials

UV/VIS Spectrophotometer
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