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Analysis of GLRX2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1016-LGZ)

Introduction

Official Full Name: glutaredoxin 2
Also known as: GRX2; CGI-133
The protein encoded by this gene is a member of the glutaredoxin protein family, which maintains cellular thiol homeostasis. These proteins are thiol disulfide oxidoreductases that use a glutathione binding site and one or two active cysteines on the active site. This gene produces multiple isoforms through alternative splicing, one of which is ubiquitously expressed and localized to mitochondria, plays a role in mitochondrial redox homeostasis, and is important for protection against and recovery from oxidative stress. Other isoforms, with more stringent expression patterns, display cytoplasmic and nuclear localization, are thought to play a role in cellular differentiation and transformation, and possibly in tumor progression.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements
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