Analysis of HBG2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2238-LGZ)

Introduction

Official Full Name: hemoglobin subunit gamma 2
Also known as: TNCY; HBG-T1
The gamma-globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. The two gamma chains and the two alpha chains together make up fetal hemoglobin (HbF), which is normally replaced at birth by adult hemoglobin (HbA). In some beta-thalassemias and related diseases, gamma chain production continues into adulthood. These two types of gamma chains differ at residue 136, where glycine is present in the g-gamma product (HBG2) and alanine is present in the a-gamma product (HBG1). The former predominates at birth. The order of the genes in the beta-globin cluster is: 5'- epsilon -- gamma-G -- gamma-A -- delta -- beta--3'.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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