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Analysis of HSP27/HspB1 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0256-LGZ)

Introduction

Heat shock protein 27(HSP-27) is a member of the low molecular weight heat shock protein family. Hsp27 is an important protein involved in drug resistance, cell growth, apoptosis, tumor occurrence and metastasis, etc. These functions mediated by Hsp27 may be related to its effects on other proteins. When the cells were not stressed, the expression of Hsp27 was low and mainly presented in large polymers, and Hsp27 under such polymers generally had no activity. When cells are stressed, Hsp27 expression is increased and phosphorylated, making Hsp27 active and performing some of its functions.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Muscle Injury, Toxicology

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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