Analysis of IL-6R (Human) by ELISA (CAT#: STEM-MB-0996-LGZ)

Introduction

Interleukin-6 receptor (IL-6R) is the receptor for IL-6 and belongs to the type I cytokine receptor family, subfamily 3. IL-6 is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in the immune response. IL-6R is a protein complex consisting of this protein and the interleukin 6 signal transducer (IL6ST/GP130/IL6β), a receptor subunit also shared by many other cytokines. Dysregulated production of IL-6 and this receptor has been implicated in the pathogenesis of many diseases such as multiple myeloma, autoimmune diseases and prostate cancer.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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