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Analysis of Influenza B NP (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0368-LGZ)

Introduction

Influenza A and B are two influenza viruses that cause epidemics in humans. Currently circulating influenza B viruses are antigenically and genetically divided into two distinct groups: Victoria and Yamagata lineages. Influenza B viruses are a genus of the family Orthomyxoviridae. The limited host range means that influenza B virus pandemics are rare. Nucleoproteins protect negative-strand viral RNA from nucleases by encapsulating negative-strand viral RNA. The encapsulated genomic RNA is called ribonucleoprotein (RNP) and serves as a template for transcription and replication.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Neurobiology/Neurodegeneration

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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