Analysis of KCNJ10 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1082-LGZ)

Introduction

Official Full Name: potassium inwardly rectifying channel subfamily J member 10<br />Also known as: KIR1.2; KIR4.1; SESAME; BIRK-10; KCNJ13-PEN<br />The gene encodes a member of a family of inward rectifier-type potassium channels that are characterized by a greater preference for allowing potassium to flow in rather than out of the cell. The encoded protein may form a heterodimer with another potassium channel protein and may be responsible for potassium buffering in glial cells in the brain. Mutations in this gene are associated with susceptibility to seizures in a common idiopathic generalized epilepsy syndrome.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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