Analysis of LCE2C Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1115-LGZ)
Introduction
Official Full Name: late cornified envelope 2C Also known as: LEP11 Enables identical protein binding activity. Predicted to be involved in keratinization.
Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.
Applications
Gene mutation analysis.
Procedure
1. Sample processing and preparation of PCR reaction system. 2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument. 3. Set the program for PCR amplification. 4. Data analysis.
Materials
Sample: depends on the customer's analysis requirements