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Analysis of LDB3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2073-LGZ)

Introduction

Official Full Name: LIM domain binding 3<br />Also known as: MFM4; ZASP; CMD1C; CMH24; CMPD3; LVNC3; CYPHER; LDB3Z1; LDB3Z4; ORACLE; PDLIM6<br />This gene encodes a protein containing a PDZ domain. PDZ motifs are modular protein-protein interaction domains consisting of 80-120 amino acid residues. Proteins containing PDZ domains interact in cytoskeletal assembly or with other proteins involved in membrane protein targeting and clustering. The protein encoded by this gene interacts with α-actin 2 through its N-terminal PDZ domain and with protein kinase C through its C-terminal LIM domain. The LIM domain is a cysteine-rich motif defined by 50–60 amino acids comprising two zinc-binding modules. This protein also interacts with all three members of the myozenin family. Mutations in this gene are associated with fibromyalgia and dilated cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been identified; all isoforms have an n-terminal PDZ domain, whereas only the longer isoforms (1, 2, and 5) have a c-terminal LIM domain.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements