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Analysis of LOC106783501 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1591-LGZ)

Introduction

Gene description: conserved acetylation island sequence C08 enhancer
Gene type: biological region
This region, which is conserved between human and pufferfish and which overlaps the 5' ends of both the DUSP28 (dual specificity phosphatase 28) and opposite strand ANKMY1 (ankyrin repeat and MYND domain containing 1) genes, can function as an enhancer in human Jurkat T cells. Its chromatin structure represents a histone acetylation island in human T cells based on the diacetylation of histone H3 at lysines 9 and 14.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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