Analysis of LRRK2 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0388-LGZ)

Introduction

Leucine-rich repeat kinase 2 (LRRK2), also known as dardarin, is a protein kinase encoded by the PARK8 gene. The protein is mainly distributed in the cytoplasm, but also in the outer mitochondrial membrane. Mutations in the LRRK2 protein cause Parkinson's and Crohn's disease.

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Principle Applications Procedure Materials Notes Related Products

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Neurobiology/Neurodegeneration

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum

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