Analysis of MIF (Human) by ELISA (CAT#: STEM-MB-1052-LGZ)

Introduction

Macrophage migration inhibitory factor (MIF), also known as glycosylation inhibitory factor (GIF), L-dopaxel isomerase or phenylpyruvate isomerase, is a protein encoded by the MIF gene. It is an important regulator of innate immunity. CD74 is the surface receptor for MIF. Bacterial antigens stimulate leukocytes to release MIF into the bloodstream. Circulating MIF binds to CD74 on other immune cells, triggering an acute immune response. Therefore, MIF is classified as an inflammatory cytokine. In addition, glucocorticoids also stimulate white blood cells to release MIF, so MIF partially counteracts the suppressive effect of glucocorticoids on the immune system.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: plasma, serum, supernatant

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