Analysis of MIR214 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0447-LGZ)

Introduction

Official Full Name: microRNA 214
Also known as: MIRN214; mir-214; miRNA214
microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Peste Des Petits Ruminants Virus (PPRV) by Real-Time PCR Method (CAT#: STEM-MB-3687-LGZ)
Analysis of Tick-borne Encephalitis Virus (TBEV) by Real-Time PCR Method (CAT#: STEM-MB-3849-LGZ)
Analysis of Giardia Spp. by Real-Time PCR Method (CAT#: STEM-MB-3532-LGZ)
Analysis of Penicillium Purpurogenum by Real-Time PCR Method (CAT#: STEM-MB-3036-LGZ)
Analysis of Nocardia Farcinica by Real-Time PCR Method (CAT#: STEM-MB-3310-LGZ)
Analysis of Rhizoma Pinelliae by Real-Time PCR Method (CAT#: STEM-MB-4000-LGZ)
Analysis of EXT2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2460-LGZ)
Analysis of Mycoplasma Galliscepticum by Real-Time PCR Method (CAT#: STEM-MB-3732-LGZ)