Analysis of MRE11 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2447-LGZ)

Introduction

Official Full Name: MRE11 homolog, double strand break repair nuclease
Also known as: ATLD; HNGS1; MRE11A; MRE11B
This gene encodes a nucleoprotein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. The protein itself has 3'~5' exonuclease activity and endonuclease activity. This protein forms a complex with the RAD50 homologue; this complex is required for non-homologous joining of DNA ends with increased single-stranded DNA endonuclease and 3' to 5' exonuclease activity. Binding to DNA ligase, this protein facilitates the joining of non-complementary ends in vitro by exploiting short homology near the ends of DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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