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Analysis of MTARC2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0467-LGZ)

Introduction

Official Full Name: mitochondrial amidoxime reducing component 2<br />Also known as: MARC2; MOSC2<br />The protein encoded by this gene is an enzyme found in the outer mitochondrial membrane that reduces N-hydroxylated substrates. The encoded protein takes molybdenum as cofactor, cytochrome b5 type B and NADH cytochrome b5 reductase as cofactors. One of the substrates used is an N-hydroxynucleotide-based analogue, which can be toxic to cells. Other substrates include N(omega)-hydroxy-L-arginine (NOHA) and amidoxime prodrugs, which are activated by the encoded enzyme. Multiple transcript variants of this gene have been found encoding different isoforms.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements