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Analysis of NPY (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0463-LGZ)

Introduction

Neuropeptide Y (NPY) is a hormone that widely exists in the central and peripheral and maintains homeostasis. In the center, NPY has anti-anxiety and anti-epileptic functions, and has the functions of inhibiting reproduction, inhibiting muscle excitation, and inhibiting sympathetic excitation, resulting in a decrease in blood pressure, heart rate, and metabolism of the human body. It can also promote appetite, and thus become the target of dieting drugs . However, peripheral NPY has a positive stimulatory effect, and it enhances the stress response together with glucocorticoids and catecholamines. NPY can induce vasoconstriction and vascular smooth muscle proliferation in the periphery, leading to elevated blood lipids, glucose tolerance, and the release of adipocytokines.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Neurobiology/Neurodegeneration, Metabolic

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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