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Analysis of NT-proANP (Rat) by ELISA (CAT#: STEM-MB-1100-LGZ)

Introduction

Cardiovascular markers refer to markers that can be sideloged in circulating blood and can sensitively and specifically reflect myocardial injury (abnormal) and its severity. Cardiovascular markers, which normally exist mainly or only in the heart (myocardium) and are released by the heart in large quantities in the case of cardiac or cardiovascular abnormalities, can be used for screening, diagnosis, prognosis assessment and follow-up treatment of a series of circulatory diseases involving the heart, blood vessels (arteries, veins, and microvessels). The markers of cardiac function include type A natriuretic peptide (ANP), type B natriuretic peptide (BNP) and its corresponding non-bioactive complex component (NT-proANP, NT-proBNP).




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cardiac Biomarkers, Cardiac Injury, Natriuretic Peptide, Toxicology, Cardiovascular

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
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